Detection of bcr-abl fusion gene mRNA level in K562/A02 cell line by real-time quantitative RT-PCR / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 40-43, 2011.
Article
em Zh
| WPRIM
| ID: wpr-244989
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to quantitatively analyze the mRNA level of bcr-abl fusion gene in K562/A02 cell line by real-time quantitative reverse transcriptase polymerase chain reaction (RQ-RT-PCR) technique. After being cultured for a period of time, the K562/A02 cell line was collected and RNA was extracted using TRIzoL kit. The real-time quantitative reverse transcriptase polymerase chain reaction technology was used to detect the level of bcr-abl fusion gene and internal reference abl gene. The results showed that a fine reproducibility was obtained between 10(7) and 10(3) copies/ml, reproducible sensitivity of RQ-RT-PCR was 10(-5). The expression of bcr-abl fusion gene in K562/A02 cells was higher and the level of bcr-abl mRNA was more than 100% in K562/A02 cells. It is concluded that RQ-RT-PCR is a reliable, sensitive and reproducible method for detecting mRNA level of bcr-abl fusion gene, which may be useful in monitoring the chronic myeloid leukemia.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
RNA Mensageiro
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Proteínas de Fusão bcr-abl
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Sensibilidade e Especificidade
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Células K562
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Reação em Cadeia da Polimerase em Tempo Real
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Genética
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Métodos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2011
Tipo de documento:
Article