Nested coamplification polymerase chain reaction / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a simple and reliable method for intensifying the hybridization signals of gene chips.</p><p><b>METHODS</b>The authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip.</p><p><b>RESULTS</b>With the use of EDTA and another FAM-labeled probe, the hybridization signals increased by 6 times or greater.</p><p><b>CONCLUSION</b>Adding EDTA and another probe to the normal PCR products is a simple and efficient method to intensify the hybridization signal of chips.</p>