Construction and tumorigenic study on a novel fusion gene AML1-MTG16 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 271-275, 2002.
Article
em Zh
| WPRIM
| ID: wpr-245320
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To test whether splicing overlapping extension(SOE) method can be a tool for obtaining rare fusion gene's transcripts and to study the tumorigenic capacity of a novel fusion gene AML1-MTG16.</p><p><b>METHODS</b>SOE method was used to obtain AML1- MTG16 fusion gene's transcripts. MTG16, AML1-MTG16 and AML1-MTG16 without III,VI conserved domains of MTG16 segment were inserted into pEGFP- C1,pDsRed-N1 vector respectively,then transfected NIH3T3 cell line by lipofection. Forty-eight hours later, the transfected cells were examined by laser-scanning confocal microscopy. Stable transfected cells were obtained by G418 500ug/ul selection for one month. Growth curve, soft agar colonies formation tumorigenesis in nude mice were done to compare the difference between stable transfected cells.</p><p><b>RESULTS</b>Recombined AML1-MTG16 by SOE contained its CDS. NIH3T3 expressing AML1-MTG16 had a faster proliferation in medium, colony growth in soft agar. AML1-MTG16 expression cells also induced tumors formation following injection into nude mouse. MTG16,AML1-MTG16 and AML1-MTG16 without III,VI conserved domains of MTG16 were colocalized in the nucleus of cotransfected NIH3T3 cells under the examination of laser-scanning confocal microscope.</p><p><b>CONCLUSION</b>SOE is an effective method to get rare fusion gene's transcripts. AML1-MTG16 plays an important role in leukemogenesis. MTG16 may also have a carcinogenic property within the AML1-MTG16 fusion gene. Carcinogenic property of AML1-MTG16 is restricted to its localization in the nuclear matrix. N terminal of MTG16 may play an important part in the carcinogenic activity of AML1-MTG16.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Plasmídeos
/
Fatores de Tempo
/
Fatores de Transcrição
/
Transplante
/
Proteínas Recombinantes de Fusão
/
Transfecção
/
Proteínas de Fusão Oncogênica
/
Divisão Celular
/
Transformação Celular Neoplásica
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2002
Tipo de documento:
Article