Role of silencing PRL-3 expression by miRNA interference in the growth of gastric cancer / 中华外科杂志
Chinese Journal of Surgery
; (12): 618-621, 2008.
Article
em Zh
| WPRIM
| ID: wpr-245543
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of silencing PRL-3 expression by miRNA interference in gastric cancer growth.</p><p><b>METHODS</b>RNA interference mediated by recombinant lentivirus expressing artificial PRL-3 miRNA was employed to knockdown PRL-3 expression in human SGC7901 gastric cancer cells. MTT assay and tumor implantation experiment were conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells and the tumor growth.</p><p><b>RESULTS</b>Transfection of recombinant lentivirus expressing artificial PRL-3 miRNA significantly suppressed the proliferation of SGC7901 cells in vitro. The implanted tumor size of the PRL-3 transfection group was (1.92 +/- 0.18) cm3, significantly smaller than those in control groups [(4.74 +/- 0.39) cm3] (P < 0.05).</p><p><b>CONCLUSIONS</b>Silencing of PRL-3 significantly suppressed the proliferation of SGC7901 cells and tumor growth in vivo. PRL-3 could be a potential therapeutic target in gastric cancer.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Neoplasias Gástricas
/
Terapêutica
/
Transfecção
/
Proteínas Tirosina Fosfatases
/
Lentivirus
/
Ensaios Antitumorais Modelo de Xenoenxerto
/
MicroRNAs
/
Interferência de RNA
/
Linhagem Celular Tumoral
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Surgery
Ano de publicação:
2008
Tipo de documento:
Article