Experimental study on effect of Buchang Naoxintong containing serum for antagonizing hypoxia-induced apoptosis of cortical neurons / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 835-838, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-245630
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Buchang Naoxintong containing serum (BNCS) for antagonizing hypoxia-induced apoptosis of primary cultured cortical neurons with the sero-pharmacological method.</p><p><b>METHODS</b>Primary cortical neurons from neonate rats (within 24 h) were cultured and induced into hypoxia model on the 7th day, which were then treated with different concentrations of BNCS. Cell apoptosis was detected qualitatively and quantitatively; and further verified by agarose gel electrophoresis through analyzing in-ternucleosomal DNA fragmentation of the neurons. Besides, neuron viability was tested by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and change of nuclear morphology was observed by Hoechst 33258 staining.</p><p><b>RESULTS</b>After treatment with BNCS, the viability of the hypoxia neurons improved with significantly reduced neuron apoptosis.</p><p><b>CONCLUSION</b>Buchang Naoxintong can protect cortical neurons from hypoxia-induced apoptosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Medicamentos de Ervas Chinesas
/
Distribuição Aleatória
/
Hipóxia Celular
/
Sobrevivência Celular
/
Células Cultivadas
/
Córtex Cerebral
/
Ratos Sprague-Dawley
/
Apoptose
/
Biologia Celular
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo
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