Effect of berberine on nitric oxide release of rat intestinal mucous microvascular endothelial cells / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 832-834, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-245631
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of berberine on the release of nitric oxide (NO) by rat intestinal mucous microvascular endothelial cells (RIMECs) cultured in vitro for exploring the pharmacological mechanism of berberine in treating intestinal disease.</p><p><b>METHODS</b>Cultured RIMECs in vitro were identified adopting immunofluorescent stain with factor VIII-related antigen. NO level in the supernatant of normal cell culture or cell culture treated with different concentrations of berberine was detected with colorimetry at the 3rd, 6th, 9th and 12th h and compared.</p><p><b>RESULTS</b>Compared with that in the normal control group, NO level increased more obviously in the berberine treated groups, and the best effect was shown in the 5 microg/mL berberine treated group.</p><p><b>CONCLUSION</b>One of the important pharmacological mechanisms of berberine might be through promoting the endogenous NO release to induce endothelium-dependent dilatation of microvascular in intestinal mucosa, thus to improve the local microcirculation of intestine.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fatores de Tempo
/
Berberina
/
Células Cultivadas
/
Colorimetria
/
Biologia Celular
/
Células Endoteliais
/
Mucosa Intestinal
/
Metabolismo
/
Óxido Nítrico
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo
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