Inhibition of hepatitis B virus replication and expression by RNA interference in vivo / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 15-18, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-245764
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the inhibitory effect of small interfering RNA (siRNA) targeting HBV C gene region on hepatitis B virus (HBV) in vivo.</p><p><b>METHODS</b>An animal model of HBV infection was developed hydrodynamically, and pcDNA3.1-HBV and siRNA were together injected into the tail vein of the BALB/c mice. HBsAg was analyzed by time-resolved immunofluorometric assay, HBV DNA was analyzed by fluorogenic quantitative PCR (FQ-PCR), HBV C-mRNA was detected by semi-quantitative RT-PCR, and viral specific proteins (HBsAg and HBcAg) in the mice livers were assayed using immunohistochemical staining.</p><p><b>RESULTS</b>In the mice, the siRNA effectively inhibited HBV replication and expression compared with the controls. The inhibitive effect of siRNA on HBV lasted at least 3 days.</p><p><b>CONCLUSION</b>These results demonstrate that RNAi can substantially inhibit HBV replication and expression in vivo.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Terapêutica
/
Replicação Viral
/
Distribuição Aleatória
/
Vírus da Hepatite B
/
Complexo de Inativação Induzido por RNA
/
RNA Interferente Pequeno
/
Genética
/
Hepatite B
/
Camundongos Endogâmicos BALB C
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS