Orthogonal experiment using SFE-CO2 in extraction of essential oil from fresh Houttuynia cordata and analysis of essential oil by GC-MS / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To optimize the extraction procedure of essential oil from H. cordata using the SFE-CO2 and analyze the chemical composition of the essential oil.</p><p><b>METHOD</b>The extraction procedure of essential oil from fresh H. cordata was optimized with the orthogonal experiment. Essential oil of fresh H. cordata was analysed by GC-MS.</p><p><b>RESULT</b>The optimize preparative procedure was as follow essential oil of H. cordata was extracted at a temperature of 35 degrees C, pressure of 15,000 kPa for 20 min. 38 chemical components were identified and the relative contents were quantified.</p><p><b>CONCLUSION</b>The optimum preparative procedure is reliable and can guarantee the quality of essential oil.</p>