Application of multiplex allele-specific PCR for authentication of Panax ginseng and P. quinquefolius / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1940-1943, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-246048
ABSTRACT
<p><b>OBJECTIVE</b>Searching and identifying SNP in Panax species and using multiplex allele-specific PCR (MAS-PCR) to authenticate P. ginseng and P. quenquefolium.</p><p><b>METHOD</b>Based on genbank database of Panax species, using DNAMAN to align the sequences, identify SNP of P. ginseng and P. quenquefolium. Design allele-specific primers for P. ginseng and P. quenquefolium, optimize the PCR reaction system including the usage amount of Taq, dNTP, primer, etc. Optimized system was performed with the total DNA of 20 different sources of P. ginseng and P. quenquefolium.</p><p><b>RESULT</b>When the annealing temperature was 66 'C, the template DNA of P. ginseng could be amplified 249 bp band whereas P. quenquefolium amplified 1 049 bp band.</p><p><b>CONCLUSION</b>The MAS-PCR have the advantages of highly specific, good reproducibility and could be identify P. ginseng and P. quenquefolium in the same PCR tube. It was a potential method to use in the molecular identification of other meteria medica.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Especificidade da Espécie
/
Marcadores Genéticos
/
Reação em Cadeia da Polimerase
/
Reprodutibilidade dos Testes
/
Classificação
/
Primers do DNA
/
Raízes de Plantas
/
DNA de Plantas
/
Polimorfismo de Nucleotídeo Único
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2006
Tipo de documento:
Artigo
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