The study of an in-house method for drug resistance genotyping testing on HIV-1 strains prevailing in China / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 66-69, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-246178
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the amplification rate and the lowestlower detection limit of an in-house HIV-1 Drug resistant (HIVDR) genotyping test.</p><p><b>METHODS</b>A total of 30 plasma samples were selected, which covered all major HIV-1 subtypes predominating prevailing in China (B', CRF07_BC, CRF01 _AE). The viral loads of the 30 selected samples were detected in triplicate by Easy Q method and the average values were taken as the viral loads of the samples. Each sample was diluted to the concentration of > 1000 copies/ml, 401-1000 copies/ml, 101-400 copies/ml, 50-100 copies/ml and < 50 copies/ml with HIV-negative plasma. After extraction of nucleic acids, RT-PCR and nested PCR amplification were performed, the efficiency of amplification of each subtype and the minimum detection limit were determined statistically based on the PCR results.</p><p><b>RESULTS</b>The viral loads of the selected samples ranged from 2.03 x 10(2)-5.92 x 10(4) copies/ml. The sample of 50-1000 copies/ml have a high amplification rate (86%).</p><p><b>CONCLUSION</b>The In-house method for HIV-1 drug resistance genotyping has a high sensitivity with a high successful amplification rate, especially in the samples with low viral load. This method can be used to the detection of drug-resistant virus and to provide scientific data to treatment options for patients.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
China
/
HIV-1
/
Classificação
/
Carga Viral
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Farmacorresistência Viral
/
Genética
/
Genótipo
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2012
Tipo de documento:
Artigo
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