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Improved expression of human rotavirus VP6 antigen in the recombinant adenoviruse by codon optimization / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 420-422, 2011.
Artigo em Chinês | WPRIM | ID: wpr-246224
ABSTRACT
<p><b>OBJECTIVE</b>To increase the recombinant adenovirus vector mediated human rotavirus VP6 gene expression through coden optimization.</p><p><b>METHODS</b>We have artificially synthesized VP6 gene of group A human rotavirus according to the human biased codon. The modified gene was transfected into 293 cells using adenovirus vector and the gene product, the respective protein was produced. The expression level of optimized gene and wild type gene was detected by Immunofluorescence (IF) and Western Blot.</p><p><b>RESULT</b>A remarkable increase of the expression level of optimized VP6 gene in comparison with the wild-type control.</p><p><b>CONCLUSION</b>The coden optimization indeed help increasing the recombinant adenovirus mediated human rotavirus gene expression, which indicated the potential application of such recombinant adenoviruses in the development of adenoviral-vectored rotavirus vaccines.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas Recombinantes / Códon / Adenoviridae / Proteínas do Capsídeo / Genética / Antígenos Virais Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Experimental and Clinical Virology Ano de publicação: 2011 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas Recombinantes / Códon / Adenoviridae / Proteínas do Capsídeo / Genética / Antígenos Virais Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Experimental and Clinical Virology Ano de publicação: 2011 Tipo de documento: Artigo