Study on ribotyping of Lyme borreliosis spirochete in Guizhou province / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 1129-1131, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-246387
ABSTRACT
<p><b>OBJECTIVE</b>To define the main genotypes in Guizhou agricultural areas by molecular epidemiologic investigation of 21 Borrelia burgdorferi sensu lato of Lyme disease spirochetes and to provide the scientific bases for formulating a preventive policy.</p><p><b>METHODS</b>Polymerase chain reaction (PCR) technique was used to amplify the 23S(rrl)-5S(rrf) intergenic spacer, and amplified products were analyzed by restriction fragment length polymorphism (RFLP) and nucleotide sequencing.</p><p><b>RESULTS</b>There were two genospecies in the strains 20 strains belong to Borrelia valaisiana, 1 strain is Borelia sp.</p><p><b>CONCLUSION</b>Borrelia valaisiana was the main genotype in Guizhou agricultural areas. The harmness of B. valaisiana to human being has been confirmed. In order to efficiently prevent the harmness of agent to the people in Guizhou agriculture areas, we should study the risk further.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimorfismo de Fragmento de Restrição
/
DNA Bacteriano
/
Doença de Lyme
/
Dados de Sequência Molecular
/
RNA Ribossômico 5S
/
RNA Ribossômico 23S
/
Sequência de Bases
/
Química
/
China
/
Reação em Cadeia da Polimerase
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Epidemiology
Ano de publicação:
2003
Tipo de documento:
Artigo
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