Your browser doesn't support javascript.
loading
Laminar flow activation of ERK5 leads to cytoprotective effect via CHIP-mediated p53 ubiquitination in endothelial cells / 대한해부학회지
Anatomy & Cell Biology ; : 265-273, 2011.
Artigo em Inglês | WPRIM | ID: wpr-24642
ABSTRACT
Atherosclerosis is readily observed in areas where disturbed flow is formed, while the atheroprotective region is found in areas with steady laminar flow (L-flow). It has been established that L-flow protects endothelial cells against endothelial dysfunction, including apoptosis and inflammation. It has also been reported that extracellular signal-regulated kinase 5 (ERK5) regulated endothelial integrity and protected endothelial cells from vascular dysfunction and disease under L-flow. However, the molecular mechanism by which L-flow-induced ERK5 activation inhibits endothelial apoptosis has not yet been determined. Transcription factor p53 is a major pro-apoptotic factor which contributes to apoptosis in various cell types. In this study, we found that 15-deoxy-Delta(12,14)-prostaglandin J2 induced p53 expression and that endothelial apoptosis was reduced under the L-flow condition. This anti-apoptotic response was reversed by the biochemical inhibition of ERK5 activation. It was also found that activation of ERK5 protected endothelial apoptosis in a C terminus of Hsc70-interacting protein (CHIP) ubiquitin ligase-dependent manner. Moreover, molecular interaction between ERK5-CHIP and p53 ubiquitination were addressed with a CHIP ubiquitin ligase activity assay. Taken together, our data suggest that the ERK5-CHIP signal module elicited by L-flow plays an important role in the anti-apoptotic mechanism in endothelial cells.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fatores de Transcrição / Prostaglandina D2 / Apoptose / Ubiquitina / Células Endoteliais / Proteína Quinase 7 Ativada por Mitógeno / Aterosclerose / Ubiquitinação / Inflamação Idioma: Inglês Revista: Anatomy & Cell Biology Ano de publicação: 2011 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fatores de Transcrição / Prostaglandina D2 / Apoptose / Ubiquitina / Células Endoteliais / Proteína Quinase 7 Ativada por Mitógeno / Aterosclerose / Ubiquitinação / Inflamação Idioma: Inglês Revista: Anatomy & Cell Biology Ano de publicação: 2011 Tipo de documento: Artigo