Experimental study on the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the secretion of vascular endothelial growth factor in acid microenvironment / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 364-366, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-246583
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the secretion of vascular endothelial growth factor (VEGF) in acid microenvironment in vitro.</p><p><b>METHODS</b>Peripheral blood mononuclear cells were extracted from healthy person's blood and cultivated to transform into monocytes/macrophages. The monocytes/macrophages were cultured with Tca8113 in acid microenvironment (pH6.6 and pH6.8) and in normal microenvironment (pH7.2). The anti-tumor effect of monocytes/macrophages against Tca8113 cells were examined by MTT assay. The expression of VEGF was detected by enzyme-link immunoassay (ELISA).</p><p><b>RESULTS</b>The anti-tumor effect of monocytes/macrophages against Tca8113 cells in acid environment was lower than in normal environment (P < 0.05). VEGF excreted by mononuclear/macrophage was significantly higher in acid microenvironment than in normal microenvironment.</p><p><b>CONCLUSION</b>Due to acid microenvironment inside tumor, the anti-tumor effect of monocytes/macrophages against tumor cell was decreased, but the secretion of VEGF was gradually increased. However, the function of monocytes/macrophages on anti-tumor need more research.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Monócitos
/
Química
/
Técnicas de Cocultura
/
Meios de Cultura
/
Linhagem Celular Tumoral
/
Fator A de Crescimento do Endotélio Vascular
/
Concentração de Íons de Hidrogênio
/
Macrófagos
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2010
Tipo de documento:
Artigo
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