Your browser doesn't support javascript.
loading
Experimental study on the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the secretion of vascular endothelial growth factor in acid microenvironment / 华西口腔医学杂志
West China Journal of Stomatology ; (6): 364-366, 2010.
Artigo em Chinês | WPRIM | ID: wpr-246583
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the secretion of vascular endothelial growth factor (VEGF) in acid microenvironment in vitro.</p><p><b>METHODS</b>Peripheral blood mononuclear cells were extracted from healthy person's blood and cultivated to transform into monocytes/macrophages. The monocytes/macrophages were cultured with Tca8113 in acid microenvironment (pH6.6 and pH6.8) and in normal microenvironment (pH7.2). The anti-tumor effect of monocytes/macrophages against Tca8113 cells were examined by MTT assay. The expression of VEGF was detected by enzyme-link immunoassay (ELISA).</p><p><b>RESULTS</b>The anti-tumor effect of monocytes/macrophages against Tca8113 cells in acid environment was lower than in normal environment (P < 0.05). VEGF excreted by mononuclear/macrophage was significantly higher in acid microenvironment than in normal microenvironment.</p><p><b>CONCLUSION</b>Due to acid microenvironment inside tumor, the anti-tumor effect of monocytes/macrophages against tumor cell was decreased, but the secretion of VEGF was gradually increased. However, the function of monocytes/macrophages on anti-tumor need more research.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Monócitos / Química / Técnicas de Cocultura / Meios de Cultura / Linhagem Celular Tumoral / Fator A de Crescimento do Endotélio Vascular / Concentração de Íons de Hidrogênio / Macrófagos / Metabolismo Limite: Humanos Idioma: Chinês Revista: West China Journal of Stomatology Ano de publicação: 2010 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Monócitos / Química / Técnicas de Cocultura / Meios de Cultura / Linhagem Celular Tumoral / Fator A de Crescimento do Endotélio Vascular / Concentração de Íons de Hidrogênio / Macrófagos / Metabolismo Limite: Humanos Idioma: Chinês Revista: West China Journal of Stomatology Ano de publicação: 2010 Tipo de documento: Artigo