Differentiation characteristics of human periodontal ligament cell population in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 185-189, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-246626
ABSTRACT
<p><b>OBJECTIVE</b>To explore the multi-differentiated capability of human periodontal ligament cell population (hPDLP), and provide a theoretical basis for the periodontal regeneration by tissue engineering technique.</p><p><b>METHODS</b>hPDLP was cultured from periodontium of human tooth by the outgrowth method. STRO-1 and CD 146 expression were investigated by flow cytometry. hPDLP was induced to odontogenic/osteogenic-like and adipogenic-like cell. The multilineage differentiation capacities of hPDLP were evaluated by alizarin red stain, oil red O stain, anti-CD146 and STRO-1 immunocytochemistry, and reverse-transcriptase polymerase chain reaction analysis.</p><p><b>RESULTS</b>hPDLP was isolated from human periodontium and most of the cells retained their fibroblastic spindle shape. hPDLP can be induced into osteoblast-like cells and adipocyte-like cells, and calcium deposition and lipid droplets were detected perspectively. And the eighth generation of hPDLP had weaker potential into adipocyte-like cells than the first passage, however, there was no difference to the aspect of calcification ability between the two passages.</p><p><b>CONCLUSION</b>hPDLP cultured in vitro can differentiate into adipocytes and osteoblasts, and the first to third passage cells may have the predominance of differentiation potential.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Ligamento Periodontal
/
Regeneração
/
Técnicas In Vitro
/
Diferenciação Celular
/
Células Cultivadas
/
Adipócitos
/
Engenharia Tecidual
/
Fibroblastos
/
Citometria de Fluxo
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2010
Tipo de documento:
Artigo
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