WISP-1: a novel mediator of hepatitis B virus-related hepatocellular carcinoma / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 285-289, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-246693
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of hepatitis B virus (HBV) X protein (HBX) on expression of the host gene Wnt induced secreted protein-1 (WISP-1) that is related to the pathogenic process of hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>Tumor and paratumor tissues were collected from HCC patients, and normal liver tissues were collected from healthy controls. Immunohistochemistry was used to evaluate the in vivo presence and expression levels of HBX and WISP-1 in the three tissue types. HepG2 cells stably transfected with pc-DNA3.1(+)-HBX or with pc-DNA3.1(+) only (G0, control) were generated and used to examine in vitro the HBX-induced changes in WISP-1 expression at the mRNA and protein levels by reverse transcription polymerase chain reaction and western blotting, respectively.</p><p><b>RESULTS</b>The HCC tissues showed significantly higher rates of positivity for WISP-1 expression than the non-tumor controls (76.6% vs. paratumor 23.4% or normal tissues 0%, x2= 35.967, P less than 0.01). HBX increased WISP-1 expression in HepG2 cells at both the mRNA (1170.33 +/- 41.26 vs. G0 265.34 +/- 27.47, t = 31.63, P less than 0.01) and protein (240.33 +/- 11.37 vs. G0 40.33 +/- 7.09, F = 600.57, P less than 0.01) levels.</p><p><b>CONCLUSION</b>HBV may up-regulate expression of the host gene WISP-1 through its X protein and thus promote the development of HCC.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vírus da Hepatite B
/
Carcinoma Hepatocelular
/
Proteína Wnt1
/
Hepatite B
/
Neoplasias Hepáticas
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2013
Tipo de documento:
Artigo
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