Role of miR137-MITF in Prognostic Analysis of Multiple Myeloma / 中国实验血液学杂志

ABSTRACT

<p><b>UNLABELLED</b>Objectiive：To explore the effect of miR137 target gene MITF on the prognosis of multiple myeloma (MM).</p><p><b>METHODS</b>The target genes of miR137 were predicted by software, the GFP analysis was carried out for detecting MITF as the prognosis of multiple myeloma. The cell line overexpressing miR137 in MM cell line was constructed. Real-time qPCR and Western blot were used to detect the expression of MITF in this cell line.</p><p><b>RESULTS</b>The target genes of miR137 were MITF, BUE2H, SH3BP5 and KLF12. High expression of MITF in MM patients showed a good prognosis according to GFP analysis, but no significant difference was detected between the different subgroups. MITF expression was higher in MM cell line that over expressed miR137.</p><p><b>CONCLUSION</b>The miR137-MITF is an important index in judging the prognosis of multiple myeloma.</p>