Effects of gestational isoflurane exposure on postnatal memory and learning in rats / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 54-59, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-247184
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of gestational isoflurane exposure on postnatal memory and learning and growth-associated protein-43 (GAP-43), neuropeptide Y(NPY) expression in the hippocampus of pups.</p><p><b>METHODS</b>Twelve maternal Sprague-Dawley rats at gestation d 18(E18) were randomly divided into isoflurane group (n=6) and control group (n=6). Rats in isoflurane group were exposed to 1.3 % isoflurane for 6 h. For control group, animals breathed in 30 % oxygen and air mixed gas at the same condition. Spatial learning and memory of the offspring were determined with the Morris Water Maze(MWM) after postnatal 4 weeks. The changes of GAP-43 and NPY expression in the hippocampal CA1 region of the pups were determined by immunohistochemistry.</p><p><b>RESULTS</b>In MWM training, the escape latency to platform of the pups in isoflurane group was significantly longer, and the time spent in the third quadrant and times of original platform crossing were less than those of control animals (P<0.05). The number and optical density of GAP-43 and NPY positive neurons in the hippocampus of pups decreased significantly in the isoflurane group compared with the controls (P <0.01).</p><p><b>CONCLUSION</b>Isoflurane exposure in pregnant rats significantly impairs the spatial memory and learning of their pups at a juvenile age, which may be associated with the down-regulation of GAP-43 and NPY in the hippocampus.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Efeitos Tardios da Exposição Pré-Natal
/
Neuropeptídeo Y
/
Ratos Sprague-Dawley
/
Aprendizagem em Labirinto
/
Proteína GAP-43
/
Hipocampo
/
Isoflurano
/
Metabolismo
Limite:
Animais
/
Gravidez
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2012
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS