Identification of the lignans components after oral administration of fuzheng huayu decoction in rat serum by HPLC-MSn / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 854-858, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-247374
ABSTRACT
<p><b>OBJECTIVE</b>To study the lignans components in rat serum after oral administration of Fuzheng Huayu decoction (FZHY), and to investigate the active ingredients in vivo.</p><p><b>METHOD</b>A rapid, sensitive and selective method using liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MSn) was established. The serum samples were extracted with ethyl acetate (EtOAc)for three times. The chromatographic separation was achieved on a Waters Atlantis T3 column by gradient elution using methanol and water containing 5 mmol x L(-1) ammonium acetate as mobile phase, at a flow rate of 0.2 mL x min(-1). Mass spectra were acquired in positive ion mode. Identification and structural elucidation of the components in FZHY and dosed serum were performed by comparing their retention time and MSn spectra with those of reference compounds and reported data in the literatures.</p><p><b>RESULT</b>Schisandrin, schisandrol B, schisantherin A and schisandrin B were found in FZHY and dosed serum, but schisandrin C and deoxyschizandrin were only found in FZHY.</p><p><b>CONCLUSION</b>Schisandrin, schisandrol B, schisantherin A and schisandrin B can be directly absorbed into the blood after oral administration of FZHY, and the four lignans components from Schisandra chineisis might play a key role as the ingredient basement of FZHY for anti-liver fibrosis.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Espectrometria de Massas
/
Sangue
/
Análise Química do Sangue
/
Medicamentos de Ervas Chinesas
/
Química
/
Administração Oral
/
Cromatografia Líquida de Alta Pressão
/
Ratos Wistar
/
Lignanas
/
Métodos
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2011
Tipo de documento:
Artigo
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