Molecular identification of one Uncaria plant / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>In order to identify a species of Uncaria, molecular phylogenetic analysis was carried out by using the rDNA ITS sequence as molecular marker.</p><p><b>METHOD</b>Total DNA was extracted from the plant with modified CTAB method and thereby rDNA ITS regions were amplified with universally conserved primer. The rDNA ITS amplicon was characterized by cloning, sequencing, blasting in GenBank and phylogenetic analyses using PAUP by maximum parsimony (MP) criteria.</p><p><b>RESULT</b>The rDNA ITS entire sequence of this species of Uncaria was 719 bp. The sequence is related to the U. sinensis available in GenBank and the similarity reaches 99.7%.</p><p><b>CONCLUSION</b>Based on molecular biology methods of rDNA ITS region analysis, molecular identification is available in accurate classification on this species of Uncaria.</p>