Effects of silencing chloride intracellular channel 1 gene expression on the proliferation and invasion of mouse hepatocellular carcinoma cell lines / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 131-135, 2010.
Article
em Zh
| WPRIM
| ID: wpr-247578
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of silencing CLIC1 gene expression on the proliferation and invasion of Hca-F cells.</p><p><b>METHODS</b>The mouse CLIC1 cDNA sequence was retrieved from NCBI. Three shRNA sequences were designed and cloned into pGPU6/GFP/Neo plasmids. The plasmids were transfected into Hca-F cells with Lipofectamine 2000. Cell Counting-8 (CCK-8) kit and transwell chamber were used to study the effects of CLIC1 on the proliferation and invasion of Hca-F cells.</p><p><b>RESULTS</b>The pGPU6/GFP/Neo-shRNA-3 plasmid effectively repressed the expression of CLIC1 mRNA. Inhibition of CLIC1 gene expression led to decreased cell proliferation and reduced invasion.</p><p><b>CONCLUSION</b>CLIC1 is essential for the proliferation and invasion of Hca-F cells.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
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Plasmídeos
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RNA Mensageiro
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Transfecção
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Regulação Neoplásica da Expressão Gênica
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Canais de Cloreto
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Carcinoma Hepatocelular
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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RNA Interferente Pequeno
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Interferência de RNA
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2010
Tipo de documento:
Article