Association of polymorphisms of miRNA biogenesis related genes DICER and DROSHA with azoospermia / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To assess the association of polymorphisms of miRNA biogenesis related genes DICER and DROSHA with azoospermia.</p><p><b>METHODS</b>For 330 patients with primary azoospermia and 282 fertile male controls, single nucleotide polymorphisms (SNPs) of DICER rs3742330 and DROSHA rs10719 were determined with a restriction fragment length polymorphism (RFLP) method.</p><p><b>RESULTS</b>For the SNP rs3742330, the frequency of A allele was higher among azoospermia patients compared with the controls (72.0% vs.64.4%, P=0.004), and so was the frequency of AA genotype (53.0% vs. 41.8%, P=0.027, OR=1.829, 95%CI 1.071-3.124). On the other hand, the allelic and genotypic frequencies of rs10719 did not differ between the two groups (all P > 0.05).</p><p><b>CONCLUSION</b>Polymorphisms of rs3742330 of the DICER gene, particularly the AA genotype, may be associated with azoospermia.</p>