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Minocycline protects retinal ganglion cells after optic nerve crush injury in mice by delaying autophagy and upregulating nuclear factor-κB2 / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 1749-1754, 2014.
Artigo em Inglês | WPRIM | ID: wpr-248117
ABSTRACT
<p><b>BACKGROUND</b>Currently, no medicine is available that can prevent or treat neural damage associated with optic nerve injury. Minocycline is recently reported to have a neuroprotective function. The aims of this study were to exarmine the neuroprotective effect of minocycline on retinal ganglion cells (RGCs) and determine its underlying mechanisms, using a mouse model of optic nerve crush (ONC).</p><p><b>METHODS</b>ONC was performed in the left eye of adult male mice, and the mice were randomly divided into minocycline-treated group and saline-treated control group. The mice without receiving ONC injury were used as positive controls. RGC densities were assessed in retinal whole mounts with immunofluorescence labeling of βIII-tubulin. Transmission electron microscopy was used to detect RGC morphologies, and Western blotting and real-time PCR were applied to investigate the expression of autophagy markers LC3-I, LC3-II, and transcriptional factors nuclear factor-κB1 (NF-κB1), NF-κB2.</p><p><b>RESULTS</b>In the early stage after ONC (at Days 4 and 7), the density of RGCs in the minocycline-treated group was higher than that of the saline-treated group. Electron micrographs showed that minocycline prevented nuclei and mitochondria injuries at Day 4. Western blotting analysis demonstrated that the conversion of LC3-I to LC3-II was reduced in the minocycline-treated group at Days 4 and 7, which meant autophagy process was inhibited by minocycline. In addition, the gene expression of NF-κB2 was upregulated by minocycline at Day 4.</p><p><b>CONCLUSION</b>The neuroprotective effect of minocycline is generated in the early stage after ONC in mice, partly through delaying autophagy process and regulating NF-κB2 pathway.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Células Ganglionares da Retina / Autofagia / Traumatismos do Nervo Óptico / Usos Terapêuticos / Tratamento Farmacológico / Subunidade p52 de NF-kappa B / Metabolismo / Minociclina Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2014 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Células Ganglionares da Retina / Autofagia / Traumatismos do Nervo Óptico / Usos Terapêuticos / Tratamento Farmacológico / Subunidade p52 de NF-kappa B / Metabolismo / Minociclina Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2014 Tipo de documento: Artigo