Overexpression of Hath1 induces production of hair cell-like cells in greater epithelial ridge cell cultures from postnatal rat cochlea / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
;
(12): 360-363, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-248164
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Hath1 (human atonal homolog 1) overexpression on greater epithelial ridge (GER) cells from postnatal rat cochlea in vitro.</p><p><b>METHODS</b>GER cells were isolated by using a combinatorial approach of enzymatic digestion and mechanical separation from P1 rat cochlear. The GER cell cultures were infected by adenovirus containing Hath1 and enhanced green fluorescent protein (ad-Hath1-EGFP), while transfecting EGFP(ad-EGFP) was as controls. Immunostaining were performed at different time points after adenovirus infection.</p><p><b>RESULTS</b>Some of the infected GER cells became myosin VIIa-positive following ad-Hath1-EGFP infection. The earliest time point to see induction of hair cell differentiation (hair cell marker expression) by ad-Hathl was 5 days post-infection. In contrast, infection of the GER sheet cultures with ad-EGFP control virus did not show any myosin VIIa-positive cells at 3-12 days post-infection in all cultures examined.</p><p><b>CONCLUSIONS</b>GER cells may potentially serve as hair cell progenitors and they are capable of differentiating hair cell-like cells when forced to express Hath1.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transfecção
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Diferenciação Celular
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Células Cultivadas
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Ratos Sprague-Dawley
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Cóclea
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Biologia Celular
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Células Epiteliais
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Fatores de Transcrição Hélice-Alça-Hélice Básicos
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Genética
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Células Ciliadas Auditivas
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2008
Tipo de documento:
Artigo
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