Gene expression of methylene tetrahydrofolate reductase and its silencing effect in primary culture mouse embryo palatal mesenchymal cell / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 244-247, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-248260
ABSTRACT
<p><b>OBJECTIVE</b>To construct a small interfering RNA (siRNA) eukaryotic expression vector specific for methylene tetrahydrofolate reductase (MTHFR) gene and to observe its silencing effect on MTHFR gene.</p><p><b>METHODS</b>The expression vectors of PsiRNA-MTHFR were constructed by gene recombination and then were nucleofected into the primary cultured MEPM cell. At 48 h and 5 d after nucleofection, the expression of MTHFR in the levels of mRNA and protein was detected by real-time quantitative polymerase chain reaction (Real-Time PCR) and Western blot.</p><p><b>RESULTS</b>The eukaryotic expression vector of PsiRNA-MTHFR, which significantly down-regulated mRNA and protein of MTHFR at 48 h and 5 d after nucleofection, were successfully constructed.</p><p><b>CONCLUSION</b>Eukaryotic expression vector of siRNA specific for MTHFR is successfully contructed, which lays the basis for its application in the mechanism research of MTHFR gene regulating embryo palate shelves fusion.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Transfecção
/
Células Cultivadas
/
RNA Interferente Pequeno
/
Metilenotetra-Hidrofolato Redutase (NADPH2)
Limite:
Animais
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2009
Tipo de documento:
Artigo
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