Molecular cloning of TSARG3 gene related to apoptosis in human spermatogenic cells / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To clone a novel gene which is related to human testis spermatogenesis apoptosis.</p><p><b>METHODS</b>To rapidly attain human novel gene full-length cDNA sequence from a human testis cDNA library,the gene-specific primers and the vector-specific primers were designed for nested polymerase chain reaction. Sequencing was performed and the result was analysed.</p><p><b>RESULTS</b>The present authors discovered the TSARG3 gene(GenBank accession number AF419291) from a human testis cDNA library, using a cDNA fragment (GenBank accession number BE644537) as an electronic probe, which was significantly changed in cryptorchidism and represented a novel gene. Furthermore, a mouse homologue of this gene was identified (GenBank accession number AF419292) by using the same method.</p><p><b>CONCLUSION</b>A novel gene named TSARG3 was cloned. It is considered that the function of the new gene is related to human testis spermatogenesis apoptosis.</p>