Sex determination of human preimplantation embryo using nested polymerase chain reaction / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 64-65, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-248497
ABSTRACT
<p><b>OBJECTIVE</b>Using nested polymerase chain reaction (PCR) to perform preimplantation gender diagnosis.</p><p><b>METHODS</b>One (or two) lymphocyte and blastomere (n=50/group) were collected and prepared under the following conditions (1) water only (H(2)O); (2) freeze-thaw liquid nitrogen, then boiling; (3) potassium hydroxide/dithiotheriol, heated to 65 degree centigrade, followed by acid neutralization (KOH). Cells were analyzed by PCR using nested primers amplification with amelogenin gene.</p><p><b>RESULTS</b>The amplification rate and allele dropout (ADO) rate for male lymphocytes by the three methods were 83%, 94%, 95% and 24%, 12%, 4%, respectively. Using two cells per reaction did not increase the amplification rate for the KOH method.</p><p><b>CONCLUSION</b>The KOH method for DNA preparation is superior to the other methods evaluated. Dual blastomere biopsy and independent blastomere analysis may improve preimplantation diagnostic reliability.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Análise para Determinação do Sexo
/
Blastocisto
/
Blastômeros
/
DNA
/
Linfócitos
/
Reação em Cadeia da Polimerase
/
Biologia Celular
/
Proteínas do Esmalte Dentário
/
Amelogenina
/
Genética
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2003
Tipo de documento:
Artigo
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