Identification of AP1 cis-element and transcriptional effect on cytokeratin 13 gene expression / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 479-483, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-248524
ABSTRACT
<p><b>OBJECTIVE</b>To identify the type of CTGAATCA from -nt.199 to -nt.192 of the cytokeratin 13(CK13) gene 5' flanking region and determine its transcriptional effect on CK13 gene expression.</p><p><b>METHODS</b>The CAT systems were used to assess the effects of different motifs of CK13 gene 5' flanking region on transcription. The clones of pCAT-enhancer with the total length, -nt.207 to +nt.63 and the same length of -nt.207 to +nt.63, but the T, G of -nt.198, -nt.197 being changed to A, T of the CK13 gene 5' flanking region, were constructed and transferred to HeLa cells with the help of lipofectin. Then work was done to detect the instant CAT expression of different clones and evaluate the effects of CTGAATCA of the 5' flanking region on CK13 gene expression. The type of the cis-element of CTGAATCA was identified with electrophoretic mobility shift assay (EMSA) and competition-EMSA.</p><p><b>RESULTS</b>CTGAATCA in the CK13 gene 5' flanking region is an AP1 cis-element by EMSA and competition-EMSA, it promotes CK13 gene expression.</p><p><b>CONCLUSION</b>CTGAATCA from -nt.199 to nt.192 of the CK13 gene 5' flanking region is an AP1 reaction element, not a cAMP reaction element. It promotes transcriptional activity of CK13 gene 5' flanking region.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transcrição Gênica
/
Ligação Competitiva
/
Sítios de Ligação
/
Proteínas Recombinantes de Fusão
/
DNA
/
Sequência de Bases
/
Células HeLa
/
Transfecção
/
Cloranfenicol O-Acetiltransferase
/
Regulação da Expressão Gênica
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2002
Tipo de documento:
Artigo
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