Cloning of human brain-derived neurotrophin-6 gene and its expression in procaryotic cell / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 475-478, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-248525
ABSTRACT
<p><b>OBJECTIVE</b>To clone human brain-derived neurotrophin-6(NT-6) gene and to observe its expression in the procaryotic cell.</p><p><b>METHODS</b>Total RNA was extracted from aborted antenatal cerebral cortex, and cDNA fragment of NT-6 was amplified through reverse transcript-polymerase chain reaction. After being incised and recovered, the NT-6 gene was cloned into pBK-CMV plasmid to construct a NT-6 gene expression vector. Expression of NT-6 gene in Escherichia coli was studied after being induced by isopropyl beta-D-thiogalactoside(IPTG).</p><p><b>RESULTS</b>The NT-6 gene expression vector was constructed and Escherichia coli with recombinant vector expressed specific protein after induction by IPTG.</p><p><b>CONCLUSION</b>The cloning of human brain-derived NT-6 gene provides a basis for further studying the structure, function and clinical application of NT-6.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Encéfalo
/
Proteínas Recombinantes
/
RNA
/
Dados de Sequência Molecular
/
Expressão Gênica
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Química
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Sequência de Aminoácidos
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Clonagem Molecular
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Homologia de Sequência de Aminoácidos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2002
Tipo de documento:
Artigo
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