Co-expression of HSV-1 gD gene and interleukin-2 gene in dendritic cell / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 328-330, 2007.
Article
em Zh
| WPRIM
| ID: wpr-248764
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct eukaryotic expression plasmid IRES-gD-IL-2 which contains both HSV-1 glycoprotein D (gD) gene and IL-2 gene and to induce it to express in antigen presenting cell (APC) -- dendritic cells (DCs).</p><p><b>METHODS</b>The whole sequence of gD and IL-2 were amplified by PCR assay. After confirmation by PCR, double-enzyme digestion and sequencing, these genes were directly cloned into eukaryotic expression vector IRES, then were transfected into DCs. Western blotting was employed to identify the transcription and expression of gD gene.</p><p><b>RESULTS</b>The results of PCR and enzyme digestion showed that the recombinant expression plasmid contained correct fragments, and the transcription and expression of gD were confirmed by Western blotting.</p><p><b>CONCLUSION</b>The recombinant expression vector IRES-gD-IL-2 was constructed, the results of the Western blotting showed that the recombinant protein could be identified by gD- specific antibody, therefore the protein has immunologic competence.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Plasmídeos
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Células Dendríticas
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Proteínas Recombinantes de Fusão
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Proteínas do Envelope Viral
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Western Blotting
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Interleucina-2
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Vacinas de DNA
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Vacinas contra o Vírus do Herpes Simples
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Alergia e Imunologia
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Genética
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2007
Tipo de documento:
Article