Effect of cytomegalovirus infection on expression of matrix metalloproteinase-2 in human endothelial cells / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 264-266, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-248783
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of cytomegalovirus infection on expression of matrix metalloproteinase-2 in human endothelial cells.</p><p><b>METHODS</b>Human umbilical vein endothelial cells were cultured in vitro. Cells between 3-6 passages were infected with cytomegalovirus for different time. Expression of matrix metalloproteinase-2 mRNA was analyzed by reverse transcription-polymerase chain reaction, matrix metalloproteinase-2 activity was detected by gel zymography.</p><p><b>RESULTS</b>Expression of matrix metalloproteinase-2 mRNA and its activity 6 hours after infection was almost equal to control, and was greatly enhanced 12 and 24 hours after infection.</p><p><b>CONCLUSION</b>Cytomegalovirus infection up-regulates expression of matrix metalloproteinase-2 in human endothelial cells. It might be one of the mechanisms that cytomegalovirus is involved in atherosclerosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Veias Umbilicais
/
Virologia
/
RNA Mensageiro
/
Regulação Enzimológica da Expressão Gênica
/
Células Cultivadas
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Metaloproteinase 2 da Matriz
/
Biologia Celular
/
Citomegalovirus
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2007
Tipo de documento:
Artigo
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