Development of an enzyme-linked immunosorbent assay for the diagnosis of lethal avian influenza virus infection / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a convenient method for diagnosis of lethal avian influenza virus infection.</p><p><b>METHODS</b>Ninety-six-well microtiter plates were coated with 100 microl of H5N1 protein (0.1 microg/ml) diluted in coating buffer. These plates were used for enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>Four of the 23 suspected borderline cases of lethal avian influenza virus infection were positive by the ELISA, and all the healthy controls (n=234) were negative. These results were confirmed by hemagglutination-inhibition (HI) and neutralization tests, and results were totally consistent (100 percent) with each other among the three methods.</p><p><b>CONCLUSION</b>The ELISA may be used in the screening for lethal avian influenza virus infection.</p>