Application of interphase fluorescence in situ hybridization in the diagnosis of lymphoma / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 733-738, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-249052
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility of interphase FISH in the routine clinicopathological practice and its values in the differential diagnosis of lymphomas.</p><p><b>METHODS</b>A total of 74 fresh tissue samples clinically suspicious of lymphoma were investigated by FISH using three probes including IgH/bcl-2, IgH/CCND1 and API2/MALT1, corresponding the translocation t(14;18), t(11;14) and t(11;18) respectively. The results of FISH were analyzed and compared with the histopathologic diagnosis.</p><p><b>RESULTS</b>Histological evaluation eventually confirmed that there were 62 cases of lymphoma and 12 cases of reactive lymphoid processes. The translocations were detected in 7 cases in 62 cases of lymphoma 3 demonstrated t(14;18) including 2 cases of follicular lymphomas and 1 nodular sclerosing Hodgkin lymphoma. Four cases had t(11;14) including mantle cell lymphoma (2 cases), follicular lymphoma (1 case) and small cell lymphoma (1 case). A lymphoid hyperplasia case showed detectable t(14;18). All 25 cases of DLBCL showed no evidence of t(14;18). Amplification or loss of regional genes was seen more often in malignant than in the benign cases.</p><p><b>CONCLUSION</b>Interphase FISH offers useful ancillary technology that plays an important role in differential diagnosis and classification of lymphoma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Translocação Genética
/
Cromossomos Humanos Par 11
/
Cromossomos Humanos Par 14
/
Cromossomos Humanos Par 18
/
Linfoma não Hodgkin
/
Doença de Hodgkin
/
Leucemia Linfocítica Crônica de Células B
/
Linfoma Folicular
/
Linfoma Difuso de Grandes Células B
Tipo de estudo:
Estudo diagnóstico
Limite:
Adolescente
/
Adulto
/
Idoso
/
Aged80
/
Criança
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2009
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS