Effects of protein kinase CK2alpha on the proliferation and invasion of human nasopharyngeal carcinoma / 中华病理学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of protein kinase CK2alpha expression on the proliferation and invasion of human nasopharyngeal carcinoma and related mechanism.</p><p><b>METHODS</b>RNA interference (RNAi) was used to down-regulate the protein kinase CK2alpha expression in 5-8F cells. The biological behavior of treated cells were analyzed by MTT and in-vitro invasion assays. Cell proliferation cycle was examined by flow cytometry and the phosphorylation status of Akt protein was examined by Western blotting analysis.</p><p><b>RESULTS</b>CK2alpha protein was successfully silenced by siRNA. CK2alpha knockdown significantly decreased the proliferative and invasive abilities of 5-8F cells. Flow cytometry analysis showed that the percentage of cells increased in G(0)/G(1) phase but decreased in S phase. Moreover, the expression of phosphorylated Akt was down-regulated by CK2alpha silencing.</p><p><b>CONCLUSION</b>Protein kinase CK2alpha plays an important role in the proliferation and invasion of nasopharyngeal carcinoma, and may provide a potential therapeutic target against nasopharyngeal cancer.</p>