Oleanolic acid synergizes with cyclosporine A to prolong renal allograft survival in rats / 南方医科大学学报
Journal of Southern Medical University
;
(12): 843-846, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-249347
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the synergistic effect of oleanolic acid (OA) and cyclosporine A (CsA) on the survival of renal allografts in rats.</p><p><b>METHODS</b>Renal allograft transplantation was performed using BN rats as donors and LEW rats as recipients. Forty male LEW rats were randomized into 4 equal groups for interventions with DMSO-PBS (control), OA, CsA, or CsA+OA, starting from 1 day before transplantation. Serum creatinine levels were regularly examined, and the survival of rats were recorded. On day 5 after transplantation, CD4(+) and CD8(+) T-cell infiltration in the renal grafts was analyzed by immunohistochemistry; the concentrations of the proinflammatory cytokines (IL-1β, IFN-γ, IL-2, IL-4, and IL-17), anti-inflammatory cytokine IL-10 and chemokines (IP-10, MCP-1, MIP, and Mig) were analyzed with Luminex; the T-cell phenotypes (IFN-γ, IL-10, IL-4, and IL-17) were analyzed using ELISpot.</p><p><b>RESULTS</b>In OA+CsA group, renal allograft survival was markedly prolonged and CD4(+) and CD8(+) T cell infiltration in the graft significantly decreased as compared to other groups. A significant decrease in IL-2 was observed in OA group and OA+CsA group, especially the latter. Compared with the control group, all the 3 treated groups showed significantly decreased IL-1β, IP-10 and MCP-1, increased IL-10 levels, decreased percentages of T cells secreting IFN-γ, IL-4 and IL-17, and increased percentage of T cells secreting IL-10. The increments of serum IL-10 level and T cell percentage were more prominent in OA+CsA group than in the other two intervention groups.</p><p><b>CONCLUSIONS</b>OA and CsA synergistically ameliorate renal graft rejection and inflammation and promote allograft survival and function in rats.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ácido Oleanólico
/
Farmacologia
/
Ratos Endogâmicos Lew
/
Ratos Endogâmicos BN
/
Transplante Homólogo
/
Linfócitos T
/
Citocinas
/
Transplante de Rim
/
Ciclosporina
/
Biologia Celular
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2014
Tipo de documento:
Artigo
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