Role of Sam68 in proliferation, invasion and migration of colorectal cancer cells in vitro / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the role of Sam68 (Src-associated substrate during mitosis 68 kD) in the occurrence and development of colorectal cancer.</p><p><b>METHODS</b>Colorectal cancer cell lines with stable Sam68 over-expressing and low Sam68 expression were established to test the effect of Sam68 in the proliferation, invasion, and migration of the cancer cells using colony formation, MTT and Transwell assays.</p><p><b>RESULTS</b>SW480 and Ls174t colorectal cell lines over-expressing Sam68 showed significantly enhanced cell proliferation, invasion and migration (P<0.05). Conversely, the low Sam68 expression in SW620 and HCT116 colorectal cell lines significantly suppressed the cell proliferation, invasion and migration (P<0.05).</p><p><b>CONCLUSION</b>The expression of Sam68 can promote the proliferation, invasion and migration of colorectal cancer cells lines in vitro.</p>