Experimental studies of carcino-embryonic antigen immunosensor based on piezoelectric resonate technology of quartz crystal / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 708-712, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-249521
ABSTRACT
In order to construct a new type of piezoelectric quartz immunosensor for the determination of carcino-embryonic antigen (CEA), the sensor detection pools were consisted of plastic loops and crystals that were 10 MHz quartz AT-cut with gold coated electrodes and the immobilization of the monoclonal antibody against CEA onto gold electrode surface of the quartz crystal was accomplished via Thiol method. Then the immunosensors were used in clinical laboratory. The experimental results showed that the piezoelectric immunosensor had good response to CEA, the frequency shifts were linearly dependent on CEA concentration in the range of 1.56 to approximately 50.00 ng/ml, other antigens such as alpha fetoprotein (AFP), prostate specific antigen (PSA), human chorionic gonadotropin (hCG) did not interfere with the sensor's response. The results obtained from this method were in satisfactory accordance with those obtained by radio immunoassay(P>0.05), The correlation coefficient was 0.90. Piezoelectric immunosensor for determination of CEA has the advantage of high sensitivity, high specificity, unnecessary labeling, simple performing, rapid analysis, low cost, real-time detection and repeated use, etc. It can be used for detecting serum CEA in clinical laboratory.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Quartzo
/
Sangue
/
Imunoensaio
/
Antígeno Carcinoembrionário
/
Sensibilidade e Especificidade
/
Eletrodos
/
Alergia e Imunologia
/
Desenho de Equipamento
/
Métodos
/
Anticorpos Monoclonais
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2006
Tipo de documento:
Artigo
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