Genetic diversity and mRNA expression of F-ATPase subunit uncG gene of streptococcus mutans clinical isolates / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 1-4, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-249771
ABSTRACT
<p><b>OBJECTIVE</b>To study the genetic diversity and the gene expression of membrane-bound proton-translocating ATPase (F-ATPase) subunit gene uncG derived from Streptococcus mutans (S. mutans) clinical isolates.</p><p><b>METHODS</b>38 S. mutans strains derived from caries-active and caries-free individuals including 18 strains displaying high acid tolerance and 20 strains displaying low acid tolerance. Gene uncG was amplified with specific primers from S. mutans genomic DNA, then the PCR product was analyzed by RFLP and sequenced. The relative expression quantity of uncG gene against the housekeeping gene recA was determined by using RT-PCR method. A gel documentation system and QUANTITY ONES software were used to analyze the data results.</p><p><b>RESULTS</b>It was testified that four genotypes A, B, C and D of PCR-RFLP were revealed when respectively digested with Alu I and Bsr I, but the distributions of the four genotype strains showed no difference (P > 0.05). The differences of uncG gene transcript quantities derived from different genotype or different aciduranc strains had no significance (P > 0.05).</p><p><b>CONCLUSION</b>This study indicated that uncG gene of F-ATPase obviously displayed genetic diversity and existed polymorphism at mRNA expression level, while the Alu I-RFLP genotypes and the expression levels would not be responsive to different acid tolerance of S. mutans strains.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimorfismo Genético
/
Streptococcus mutans
/
Variação Genética
/
Polimorfismo de Fragmento de Restrição
/
RNA Mensageiro
/
Reação em Cadeia da Polimerase
/
Adenosina Trifosfatases
/
Cárie Dentária
/
Genótipo
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2007
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS