Study on the horizontal transmission of oral Streptococcus mutans in day-nursery children / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 527-529, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-249779
ABSTRACT
<p><b>OBJECTIVE</b>To analyze genotypic diversity of oral Streptococcus mutans (S. mutans) and find out horizontal transmission possibility of the microbe in day-nursery children.</p><p><b>METHODS</b>The plaque samples were scratched with sterilized toothpicks from teeth of 32 day-nursery children aged between 3 and 4, then cultured on MSB plates. Clones with representative S. mutans-like were subcultured and identified to species level biochemically. AP-PCR fingerprinting was performed to distinguish genotypic diversity of those isolates. Then S. mutans isolated from different children with very similar amplicon profiles were examined by chromosomal DNA fingerprinting analysis.</p><p><b>RESULTS</b>S. mutans were isolated in oral cavities of 78.1% children, 100% in caries and 69.6% in caries-free children. A total of 57 genotypes were identified by AP-PCR. More than one amplitypes were identified in 88% of the 25 children with S. mutans colonization. Two pair of children shared common genotypic S. mutans.</p><p><b>CONCLUSION</b>There is no evident relation between number of genotype detected and caries. The presence of matching genotypes of S. mutans among day-nursery children suggests the horizontal transmission may exist.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Streptococcus mutans
/
Dente
/
Reação em Cadeia da Polimerase
/
Cárie Dentária
/
Placa Dentária
/
Genótipo
/
Berçários para Lactentes
Limite:
Criança
/
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2006
Tipo de documento:
Artigo
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