Inhibitory effects of selenium on telomerase activity and hTERT expression in cadmium-transformed 16HBE cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 307-312, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-249850
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of sodium selenite on telomerase activity and expression of hTERT mRNA in cadmium-transformed 16HBE cells.</p><p><b>METHODS</b>Telomerase activity and expression of genes were measured after cultured cadmium-transformed 16HBE cells were exposed to sodium selenite at different doses (0.625, 1.25, 2.50, 5.00 micromol/L) for 24 hours.</p><p><b>RESULTS</b>Selenium decreased telomerase activity in cadmium-transformed 16HBE cells. There existed an obvious dose-effect relationship between the selenium concentration and these changes. The expression of hTERT and c-myc mRNA also decreased but the expression of mad1 mRNA increased after exposure to selenium for 24 hours. No difference was found in expression of hTRF1 and hTRF2 mRNA after incubated with sodium selenite for 24 hours, compared with control group.</p><p><b>CONCLUSION</b>Selenium inhibits telomerase activity by decreasing hTERT and c-myc mRNA expression and increasing mad1 mRNA expression in cadmium-transformed 16HBE cells and selenium concentration is significantly correlated with these changes.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Cádmio
/
RNA Mensageiro
/
Linhagem Celular Transformada
/
Sequência de Bases
/
Selenito de Sódio
/
Primers do DNA
/
Telomerase
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Genética
Limite:
Humanos
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2007
Tipo de documento:
Artigo
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