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Insulin expression in rats exposed to cadmium / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 295-301, 2007.
Artigo em Inglês | WPRIM | ID: wpr-249852
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the effects of cadmium exposure on insulin expression in rats.</p><p><b>METHODS</b>Eighteen adult SD rats were administered cadmium subcutaneously (0.5, 1.0, and 2.0 mg/kg x bw). The effects on endocrine of pancreas were assessed. The levels of cadmium and zinc in pancreas, blood and urine glucose, serum insulin and urine NAG (N-acyetyl-beta-glucosaminidase) were determined. The gene expressions of metallothionein (MT) and insulin were also measured, and the oral glucose tolerance tests (OGTT) were carried out.</p><p><b>RESULTS</b>The contents of cadmium in pancreas in cadmium-treated rats were higher than that in the control group, which was associated with slight increase of zinc in pancreas. Cadmium-exposed rats (1.0 and 2.0 mg/kg x bw) demonstrated a marked glucose intolerance. But the levels of serum insulin did not change significantly after cadmium administration, and the UNAG had no change in Cd-treated group. The gene expression of insulin decreased in 1.0 and 2.0 mg/kg x bw cadmium-exposed groups, compared with the control group. The expression of MT-I was higher in the groups exposed to 1.0 and 2.0 mg/kg x bw cadmium while the expression of MT-II was higher in the group exposed to 2.0 mg/kg x bw cadmium.</p><p><b>CONCLUSIONS</b>Cadmium may be accumulated in the pancreas, resulting in the change of the expression of insulin, MT-I and MT-II genes. Cadmium can influence the biosynthesis of insulin, but does not induce the release of insulin. The dysfunction of pancreas occurs earlier than that of kidney after administration of cadmium.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Urina / Sangue / Glicemia / Cádmio / Sequência de Bases / Expressão Gênica / Ratos Sprague-Dawley / Primers do DNA / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Toxicidade Limite: Animais Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Urina / Sangue / Glicemia / Cádmio / Sequência de Bases / Expressão Gênica / Ratos Sprague-Dawley / Primers do DNA / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Toxicidade Limite: Animais Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2007 Tipo de documento: Artigo