Production and characterization of monoclonal antibody against recombinant human erythropoietin / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 184-188, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-249868
ABSTRACT
<p><b>OBJECTIVE</b>To produce specific monoclonal antibody (mAb) against recombinant human erythropoietin (rHuEPO) for development of highly efficient methods for erythropoietin detection in biological fluids.</p><p><b>METHODS</b>rHuEPO was covalently coupled with bovine serum albumin (BSA) and the conjugate was used to immunize mice to produce specific mAb against rHuEPO based on hybridoma technology. The obtained F3-mAb was characterized by enzyme-linked immunosorbent assay (ELISA), SDS-PAGE and Western blot.</p><p><b>RESULTS</b>The isotype of F3-mAb was found to be IgM with an affinity constant of 2.1 x 10(8) L/mol. The competitive ELISA using the obtained IgM showed a broader linear range and lower detection limit compared with previous work.</p><p><b>CONCLUSIONS</b>The modification of rHuEPO was proved to be successful in generating required specific mAb with high avidity to rHuEPO.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes
/
Imunoglobulina G
/
Imunoglobulina M
/
Ensaio de Imunoadsorção Enzimática
/
Eritropoetina
/
Relação Dose-Resposta a Droga
/
Alergia e Imunologia
/
Camundongos Endogâmicos BALB C
/
Peso Molecular
/
Anticorpos Monoclonais
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2007
Tipo de documento:
Artigo
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