Studies of the expression, purification, renaturation and biologic activity of an anti-CEA immunotoxin / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 348-351, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-249984
ABSTRACT
A recombinant immunotoxin named CEA/PE38/KDEL was constructed, which was composed of anti-CEA single-chain Fv and the truncated and modified form of Pseudomonas exotoxin (PE38/KDEL). The CEA/PE38/KDEL immunotoxin was expressed in the E. coli strain BL21 (DE3)-star as inclusion bodies. The denatured inclusion bodies were purified with Ni-NTA chelate agarose, then the constant gradient dialysis was used to perform the refolding of the CEA/PE38/KDEL immunotoxin. Results of FACS and MTT assay indicate that the refolded immunotoxins keep potent and specific cytotoxicity to tumor cells bearing CEA antigens.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Toxinas Bacterianas
/
Proteínas Recombinantes de Fusão
/
Fragmentos de Imunoglobulinas
/
Antígeno Carcinoembrionário
/
Imunotoxinas
/
Clonagem Molecular
/
ADP Ribose Transferases
/
Renaturação Proteica
/
Fatores de Virulência
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2004
Tipo de documento:
Artigo
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