Role of CD4-CD8- T cells in the murine hepatitis virus type 3 induced chronic viral hepatitis / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 180-183, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-250019
ABSTRACT
<p><b>OBJECTIVE</b>To investigate role of CD4-CD8- T cells in murine hepatitis virus type 3 (MHV-3) induced chronic viral hepatitis in C3H/Hej mice and to identify their surface markers.</p><p><b>METHODS</b>Thirty C3H/Hej mice received 10 Pfu MHV-3 intraperitoneally, the CD4-CD8- T cells were isolated using magnetic bead sorting on 0, 4, 15, 30, 40 days post MHV-3 infection. The cytotoxic effects of CD4-CD8- T cells on normal and infected hepatocytes, CD8+ T cells and unrelated-virus (murine cytomegalovirus, MCMV) infected CD8+ T cells were examined by non-radioactive cytotoxicity assay. The surface markers of CD4-CD8- T cells were determined by flow cytometry.</p><p><b>RESULTS</b>MHV-3 infected CD4-CD8- T cells showed significant cytotoxic effect on CD8+ T cells, but not on infected hepatocytes or MCMV infected CD8+ T cells. The analysis of cell surface markers demonstrated that the CD4-CD8- T cells are a completely new T cell subset.</p><p><b>CONCLUSIONS</b>CD4-CD8- T cells have significant cytotoxic effect on virus specific CD8+ T cells in MHV-3 infected C3H/Hej mice, which suggests that CD4-CD8- T cells have immune modulatory functions in the development of chronic viral hepatitis. The phenotype of these CD4-CD8- T cells detected by flow cytometry is TCR alpha beta +CD3+CD4- CD8- CD25- CD28- CD30- CD44+.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Baço
/
Fatores de Tempo
/
Virologia
/
Linfócitos T CD4-Positivos
/
Subpopulações de Linfócitos T
/
Infecções por Coronavirus
/
Vírus da Hepatite Murina
/
Linfócitos T CD8-Positivos
/
Alergia e Imunologia
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2009
Tipo de documento:
Artigo
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