Isolation, culture and multipotent differentiation of mesenchymal stem cells from human fetal livers / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 711-713, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-250116
ABSTRACT
<p><b>OBJECTIVE</b>To isolate and culture mesenchymal stem cells (MSCs) from human fetal livers and describe their biological characteristics.</p><p><b>METHODS</b>MSCs were acquired using an optimized method. Cell cycles and the immunophenotype of the cells were analyzed by flow cytometry. The osteogenic and adipogenic differentiations were induced and identified by specific stainings, and hepatic differentiation by morphology and RT-PCR.</p><p><b>RESULTS</b>The target cells derived from human fetal livers adhered to the plate with fibroblast-like morphology, whose surface markers were CD90, CD44, CD147 positive, and CD34, CD45, HLA-DR negtive. In the differentiation study, these cells could be induced to differentiate into osteogenic, adipogenic and hepatocyte-like cells.</p><p><b>CONCLUSION</b>Multipotent MSCs can be isolated and cultured from human fetal livers.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Diferenciação Celular
/
Separação Celular
/
Células Cultivadas
/
Biologia Celular
/
Feto
/
Células-Tronco Mesenquimais
/
Fígado
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2004
Tipo de documento:
Artigo
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