A preliminary study using RNA interference technique against replication of HBV in vitro / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 519-521, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-250169
ABSTRACT
<p><b>OBJECTIVE</b>To identify the siRNA interference ability for the replication of HBV.</p><p><b>METHODS</b>Based on the sequence of HBV in HepG2 2.2.15 cells in GenBank, one sequence targeting the C antigen of HBV was cloned into the RNA polymerase III based expression vector pSuper. This recombinant was electroporated into HepG2 2.2.15 cells and the expression of HBsAg and HBeAg was assayed using ELISA.</p><p><b>RESULTS</b>The construction of the recombinant expression vector pSuper-C and its control vector pSuper was successfully confirmed by the results of enzyme digestion, electrophoresis and sequencing. However, there was no difference between the expression of HBsAg and HBeAg in the supernatant of HepG2 2.2.15 cell culture in the experimental and control groups.</p><p><b>CONCLUSIONS</b>The constructed pSuper-C did not show an interfering effect on the replication of HBV in HepG2 2.2.15 cells. In order to display this effect, further study is needed</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Replicação Viral
/
Células Tumorais Cultivadas
/
Vírus da Hepatite B
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Genética
/
Neoplasias Hepáticas
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2004
Tipo de documento:
Artigo
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