Sequence analysis of VP7 gene from rotavirus field strain from Guangzhou, China / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 165-168, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-250514
ABSTRACT
<p><b>OBJECTIVE</b>To compare the difference of VP7 fragment at the nucleotide level between rotavirus Guangzhou field strain R97-196 and rotavirus Beijing field strain or prototype strains.</p><p><b>METHODS</b>The VP7 fragment amplified from Guangzhou field strain R97-196 by RT-PCR was cloned into the T-A cloning vector pUCm-T and sequenced.</p><p><b>RESULTS</b>The VP7 fragment from Guangzhou field strain R97-196 was 1,062 bp in length and contained two open reading frames which is consistent with that reported in the literature. The sequence analysis revealed that the cDNA from R97-196 shared higher nucleotide and amino acid identities with Beijing field strain T73 (98% and %, respectively) than with serotype 2-4 (G types) rotavirus (from 74% to 77% and 73% to 81%, respectively). The divergence of amino acid sequences is mainly within the nine divergence regions. The phylogenetic analysis revealed that the VP7 fragment from R97-196 was far away from rotavirus serotype G1 strain Wa.</p><p><b>CONCLUSIONS</b>The VP7 gene fragment from rotavirus Guangzhou field strain R97-196 belongs to rotavirus serotype G1. And variation of rotavirus VP7 gene fragment seems to be a geographic matter.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Infecções por Rotavirus
/
Virologia
/
Dados de Sequência Molecular
/
Sorotipagem
/
Sequência de Aminoácidos
/
Classificação
/
Análise de Sequência
/
Rotavirus
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Humanos
/
Lactente
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2003
Tipo de documento:
Artigo
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