Detection of hantaan virus from gamasid mite and chigger mite by molecular biological methods / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 107-111, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-250529
ABSTRACT
<p><b>OBJECTIVE</b>To study the proliferation and location of hantaan virus (HV) in gamasid mites and chigger mites.</p><p><b>METHODS</b>HV RNA in gamasid mites and chigger mites were detected by reverse transcription, polymerase chain reaction (RT- PCR) and in situ hybridization.</p><p><b>RESULTS</b>The smallest quantity of mite from which HV RNA could be detected was 5 mites group. The titers of -and proliferated in mites HV RNA could be found in ovary cells and dug cells of gamasid mites and chigger mites by in situ hybridization.</p><p><b>CONCLUSIONS</b>The results showed that HV could be trans-stadially transmitted and proliferated in mites, and HV always located in ovary and dug organs of mites. These results provide direct evidence at molecular level for the role of gamasid mites and chigger mites as vectors in transmission of HV.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ovário
/
Vetores Aracnídeos
/
Virologia
/
RNA Viral
/
Chlorocebus aethiops
/
Reação em Cadeia da Polimerase
/
Hibridização In Situ
/
Vírus Hantaan
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Genética
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2003
Tipo de documento:
Artigo
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