Degradation of electrodeposited calcium phosphate and calcium phosphate-chitosan coatings in vitro / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the degradation of electrodeposited calcium phosphate (ECP) coating and calcium phosphate/chitosan (ECPC) coating in vitro.</p><p><b>METHODS</b>Osteoclasts were isolated from neonatal rabbit long bone cavities and incubated with ECP and ECPC coatings. Calcium ion concentrations in the culture medium were analyzed at 3 days and 6 days. The osteoclastic resorption was observed with scanning electron microscope.</p><p><b>RESULTS</b>Both coatings demonstrated osteoclastic resorption lacunae. The calcium ion concentrations of the culture mediums were decreased when incubated with calcium phosphate coatings (P < 0.05). Compared with coatings cultured with osteoclasts, the calcium ion concentrations of those cultured without osteoclasts were higher on day 3 (P > 0.05) but lower on day 6 (P < 0.05).</p><p><b>CONCLUSIONS</b>Both ECP and ECPC coatings can be resorbed by osteoclasts in vitro and can dissolve in the culture medium.</p>