HPLC-fluorescent spectrometric determination of serum mexiletine concentration after derivatization with fluram / 药学学报
Acta Pharmaceutica Sinica
;
(12): 215-217, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-251139
ABSTRACT
<p><b>AIM</b>To establish an HPLC-fluorescent spectrometric method for the determination of mexiletine hydrochloride in plasma after derivatization with fluram.</p><p><b>METHODS</b>Fluram acetone solution was added to the deproteinized plasma with acetone to obtain the derivative of mexiletine. The HPLC method was performed on a column of Allitima C18 (150 mm x 4.6 mm, 5 microns) with the mobile phase of methanol-water-diethylamine-phosphoric acid buffer (2.4 mol.L-1, pH 4.0) (70282), and the detective wavelength were set at Ex 392 nm and Em 480 nm.</p><p><b>RESULTS</b>Mexiletine has a liner range over the concentration range from 0.100-6.400 mg.L-1. The lowest detectable concentration of this method was 5 micrograms.L-1 (S/N > or = 4). The intra-day and inter-day RSDs were 1.34%-5.31%, respectively.</p><p><b>CONCLUSION</b>This method is simple, selective and can be used for therapeutic drug monitoring (TDM) and pharmacokinetic studies of mexiletine.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
Farmacocinética
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Fluorescamina
/
Métodos
/
Mexiletina
/
Antiarrítmicos
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2003
Tipo de documento:
Artigo
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